Lymphocyte gene rearrangements | Genomic Diagnostics

Test description

B-cell immunoglobulin heavy chain (IgH) gene rearrangements

This test is for the IgH gene rearrangements (FR1, FR2 and FR3) and will detect greater than 80% of B-cell lymphoproliferative disorders.

T-cell Receptor (beta and gamma) gene rearrangements:

This test is for detecting monoclonality of T lymphocytes using primers targeted at both beta and gamma gene rearrangements, and will detect at least 90% of T-cell lymphoproliferative disorders.

Bcl-2

This test is specific for the major breakpoint region (mbr) of the bcl-2 associated translocation [t(14;18)]. This translocation is associated with up to 85% of lymphomas with a follicular morphology, and with approximately one third of diffuse large cell lymphomas.

Bcl-1

This test is specific for the translocation involving the Cyclin D1 gene [t(11;14)]. It is commonly associated with Mantle Cell Lymphoma (MCL).

Test information

Test name

T &/or B cell rearrangement

Clinical indication

To determine clonality in lymphoproliferative disorders

Gene(s)

IGH

Method

Conventional PCR and fragment analysis

Turn around time

2 weeks

Medicare eligibility

Price

B Cell gene rearrangement: $130

T Cell gene rearrangement: $180

Test request form

Standard business unit request form

Sample type

Blood or bone marrow or Lymph node or Tumour

Collection type

Special instructions

None